Biologically inactive structural analogs of PAF are not lethal to Swiss albino mice.

Aliquots of lysoPAF and lysoPC were taken from a stock of 5 mg/mL in methanol, dried under nitrogen, reconstituted in PBS containing 0.1% albumin, and sonicated. The indicated doses of each were brought up to a total volume of 0.5 mL with PBS containing 0.1% albumin and intraperitoneally injected into the mice. Animals injected with the vehicle (PBS containing 0.1% albumin) received the same volume. PAF (5 μg/mouse) was used as a positive control; all the animals injected with PAF died within 15–20 min. No mortality was observed for the animals intraperitoneally injected with the lyso analogs at doses equivalent to that used for PAF or 10-fold higher (50 μg/mouse). All the animals were monitored for survival for up to 6 days. The results are representative of 3 individual trials.