Nanopore sequencing of cfDNA from ALL patients for IGH analysis

Clonal rearrangement of IGHV regions of Acute Lymphoblastic Leukemia (ALL) patients was analyzed by sequencing amplicons obtained from cfDNA collected at different treatment time points. The presence and prevalence of major IGHV variants identified at diagnosis were tracked throughout treatment in patients' plasma and cerebra-spinal fluid (CSF). Nanopore based analysis of cfDNA described in this research can be used as a means of surveillance for disease progression or monitoring response to therapy.