RNA-seq of Control and PRMT5-/- JHH-7 Cell Transcriptomes

Purpose: The goals of this study are to use RNA-seq-derived JHH-7 cell transcriptome profiling for differentially expressed genes after PRMT5 knockdown. Methods: mRNA profiles of control and PRMT5 knockdown JHH-7 cells were generated by RNA-seq, using Illumina Novaseq 6000.The sequence reads that passed quality filters were analyzed at the transcript isoform level with TopHat2 or HISAT2. The gene expression quantification was performed using RSEM tool and generated raw count for all genes individually. qRT–PCR validation was performed using SYBR Green assays. Results: On average, we generated >200 million paired end reads for each sample, with more than 95% of the reads passed the QC. Overall design: mRNA profiles of control and PRMT5-/- JHH-7 cells