Seq [organism=Pectobacterium carotovorum subsp. carotovorum] [clone=peh28] gene, Complete cds. Nucleotide sequence identification accession number for peh28, a clone isolated from Pectobacterium carotovorum subsp. carotovorum

The bacterium Pectobacterium carotovorum subsp. carotovorum (P. carotovorum) is notorious for its maceration of the plant cell wall causing soft rot. The ability to destroy plants is due to the expression and secretion of a wide range of hydrolytic enzymes that include cellulases and polygalacturonases. P. carotovorum ATCC™ no. 15359 was used as a source of DNA for the amplification of peh28, a gene encodes for a polygalacturonase. Primers were designed based on published gene sequences and used to amplify the open reading frames from the genomic DNA of P. carotovorum. The individual PCR products were cloned into the pTAC-MAT-2 expression vector and transformed into Escherichia coli. Molecular and biochemical studies were conducted for the assimilated clone product alongside three other clones' isolated using the same source, which could provide evidence for successful transformation of a lignocellulosic biomass oligomer into biofuel related precursors. *the clone's DNA was sequenced via Molecular Cloning Laboratories ex-facility (MCLAB, San Francisco, CA, USA). >Seq ATGGAATATCAATCAGGCAAGCGAGTTTTATCATTATCACTGGGGCTTATCGGTTTGTTT AGCGCATCGGCATTTGCATCTGATTCCCGAACGGTGAGTGAACCGAAAACACCGTCTTCC TGTACGGTGCTTAAAGCGGACAGTAGTACTGCCACGAGTACAATTCAAAAAGCGCTGAAT AATTGCGGGCAAGGAAAAGCGGTAAAGCTGAGCGCAGGGAGTTCATCCGTTTTTCTGAGT GGCCCGCTTTCTCTACCTTCTGGCGTGAGCTTATTAATCGACAAAGGAGTAACCCTACGT GCTGTGAATAATGCCAAGTCTTTTGAGAATGCGCCGTCATCCTGTGGCGTGGTGGATACA AACGGTAAAGGCTGCGATGCATTTATTACCGCTACAAGCACGACGAATAGCGGAATCTAT GGGCCGGGCACCATTGATGGACAAGAGGGCGTGAAGCTTCAGGATAAAAAGGTGAGCTGG TGGGATCTGGCTGCCGATGCCAAAGTGAAAAAGTTAAAACAGAATACCCCCCGGCTGATT CAGATTAATAAGAGCAAGAATTTCACGCTGTATAACGTCTCTCTCATTAATTCTCCGAAC TTCCACGTCGTGTTCAGCGATGGTGACGGCTTCACCGCGTGGAAAACCACGATCAAAACG CCGTCCACCGCCAGAAACACTGACGGTATCGATCCGATGTCGTCAAAGAACATCACCATT GCCCACAGTAATATTTCGACGGGCGACGATAATGTGGCGATCAAAGCCTATAAAGGTCGT TCTGAGACACGCAATATTTCCATTTTGCATAATGAATTTGGAACGGGACATGGCATGTCG ATCGGTAGCGAAACGATGGGAGTTTATAACGTGACGGTCGATGATCTGGTTATGACTGGC ACCACGAATGGATTACGTATTAAAAGCGACAAATCAGCAGCCGGCGTTGTCAATGGTGTT CGGTATAGCAACGTAGTCATGAAGAACGTGGCGAAACCGATCGTGATTGACACGGTATAT GAGAAAAAAGAGGGAAGTAATGTTCCTAACTGGAGCGACATTACGTTTAAGGATATTACG TCTCAAACCAAAGGTGTGGTGGTGCTGAACGGCGAGAATGCGAAAAAGCCGATAGAAGTG ACGATGAAGAACGTCAAACTGACGAGCGACAGCACATGGCAAATCAAGAACGTCACCGTT AAGAAATAA