Transcriptomic and epigenetic profiling of blood mTreg and mTeff from healthy control (HC) and multiple sclerosis (MS) [ATAC-Seq]

Autoimmune diseases, among the most common disorders of young adults, are mediated by genetic and environmental factors. While CD4+Foxp3+ regulatory T cells (Tregs) play a central role in preventing autoimmunity, the molecular mechanism underlying their dysfunction is unknown. Here, we performed comprehensive transcriptional and epigenetic profiling of Tregs in multiple sclerosis (MS) to identify central transcriptional programs regulating human autoimmunity. Overall design: CD4+CD25hiCD127loCD45RO+ memory Tregs and CD4+CD25low/negCD127hiCD45RO+ memory Tconvs are sorted by FACS and subjected to ATAC-seq