Enhancing radiotherapy response via intratumoral injection of the TLR9 agonist CpG to stimulate CD8 T cells in an autochthonous mouse model of sarcoma

p53/MCA sarcomas are induced by intramuscular injection of adenovirus expressing Cas9 and sgRNA targeting Trp53 (Adeno-p53-sgRNA; Viraquest) at gastrucnemius muscle site. We used bulk tumor RNAseq to analyze the differences in tumor cells and immune cells after treatment with CpG+RT we performed bulk tumor RNA-seq on an independent cohort of p53/MCA tumors harvested 6 days after RT and 3 days after CpG treatment or their respective controls. Ctl (n=5), CpG alone (n=5), RT alone (n=5), CpG+RT (n=5)