DNA methylation and transcriptome integration analysis reveals potential markers associated with the pathogenesis of type A aortic dissection [miRNA-seq]

This study aimed to identify candidate markers of TAAD by integrating methylation and transcriptome sequencing analyses. Overall design: Aortic vascular tissue samples from TAAD cases and controls were used for transcriptome sequencing and methylation analysis using the Illumina Hiseq sequencing platform and Illumina Infinium MethylationEPIC BeadChip microarray, respectively. A series of bioinformatics analyses and machine learning algorithms were used to identify key methylated genes from the differentially expressed genes and differentially methylated sites between TAAD and controls.