RNA-Seq analysis of estrogen-deprived MM134 HER2WT and HER2L755S cells

We conducted RNA sequencing of MM134 lobular cell line expressing HER2 WT and L755S cells grown in low estrogen conditions followed by Gene Set Enrichment Analysis (GSEA) to identify altered gene expression pathways in L755S cells. mTOR (also called MTORC1) signaling was the top significantly up-regulated pathway in HER2 L755S cells as compared to HER2 WT cells mRNA profiles of two different cell lines to identify altered gene expression upon estrogen deprivation.