Neutrophil-derived microvesicle induced dysfunction of the blood-brain barrier in vitro

The aim of this study is to understand how NMV can affect the vascular integrity of the blood-brain barrier using a cell line- hCMEC/D3. Recent studies have reported that NMV can affect other endothelial barriers in the body contributing to inflammation and pathogenesis of diseases such as atherosclerosis. The transcriptomic profile of NMV-treated hCMEC/D3 cells was compared to that of cells without treatment to understand whether functional groupings and pathways are differentially expressed, which can contribute to changes in blood-brain barrier. This study is to understand how NMV interact with hCMEC/D3 cells and can alter the transcriptomic profile of these cells after NMV treatment for 24h. Changes in the transcriptomic profile of the brain endothelial cell line, hCMEC/D3, after treating with neutrophil-derived microvesicles (NMV) were compared to control cells receiving no treatment, to identify differentially expressed genes and pathways.