IR DC-CM treated BMSCs

The BMSCs were cultured with unirradaited/irradiated DC-CM for 3 days. Total RNA was extracted and sent to DIATRE biological technology company (China) for library preparation, RNA-seq, and data analysis. DESeq2 package was carried out to perform differential expression analysis, and differentially expressed genes (DEGs) were determined by a P value <0.05. Gene ontology (GO) analysis and Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis were utilized to detect the molecular and biological processes as well as signaling pathways. Overall design: RNA-seq profiling of BMSCs induced by unirradiated/irradiated mDC-CM (ctrl, mDC-0Gy, mDC-8Gy) at Day3.