Mouse embryonic stem cells (mESCs) differentiation towards ventral hindbrain neuronal cell types [d4.5, d5.5 and d6.5]

mESCs treated with retinoic acid (RA) and a Shh agonist (SAG) recapitulated ventral hindbrain development and produced a population of neural progenitors with a minor presence of other cell types. The differentiation in mESCs was induced by RA and SAG in monolayer condition. The sorted cells were collected at different timepoints (i.e., d4.5, d5.5, d6.5) and processed for RNA isolation and RNA-seq.