Real-time quantitative PCR analysis of serum from osteoporotic Mexican women

RNA was purified from serum of osteoporotic and healthy postmenopausal mexican women using miRNeasy Serum/Plasma kit (QIAGEN). For microRNA expression analysis we used the Human MicroRNA A+B Cards Set v3.0 TaqMan Low Density Array platform (Applied Biosystems). Analysis was performed in the Expression Suite v.1.1.3 software (Applied Biosystems). We made 4 pools for each studied group, pooling 150 ng of RNA of 5 samples. Hence, we have 4 pools for Normal and 4 pools for osteoporotic women.