Apis cerana cerana isolate:FLBSL-2018 Genome sequencing and assembly

Apis ceranais one of the main honeybee species in artificial farming, which is widely distributed in Asian countries. The genome of A. cerana has been sequenced by several different research groups using second generation sequencing technologies. However, it is still necessary to obtain more complete and accurate genome sequences.We present a chromosome-scale assembly of the A. cerana genome using single-molecule real-time Pacific Biosciences sequencing and high-throughput chromatin conformation capture (Hi-C) genome scaffolding. The updated assembly is 215.67 Mb in size with a contig N50 of 4.49 Mb, representing an~212-fold improvement over the previous Illumina-based version. Hi-C scaffolding resulted in 16 pseudochromosomes. A total of 10,741 protein-coding genes were predicted and 9,627 genes were annotated. Besides, 314 new genes were identified compared to the previous version.The improved high-quality A. cerana reference genome will provide precise sequence information for the biological research of A. cerana.