Analysis of RNA editing sites in Adar1 mutant mice and cells

Adenosine-to-inosine RNA editing is catalyzed by ADAR1 and ADAR2 in mammals. ADAR1 has two isoforms, short p110 and long p150. Although both ADAR1 KO mice and ADAR1 KI mice that harbor the editing-inactive E861A point mutation are embryonically lethal, this lethality can be rescued by concurrent deletion of MDA5. To elucidate the domains critical for ADAR1-mediated RNA editing, we generated Adar1 knock-in mice and compared RNA editing patterns in brain and colon with wild-type mice. In addition, we generated Adar1/Adar2 double knockout Raw cells and forcedly expressed mutated Adar1. Then, RNA editing pattern was compared among mutants and wild-type ADAR1.