Analysis on fungal community structure of piggery wastes treated by microbial nest technology

High throughput sequencing of its1-its2 region of fungal its gene hypervariable region was analyzed by Illumina Miseq sequencing platform. According to the changes of Ace index, Chao1 index, Shannon index, Simpson index, Smithwilson index and Coverage index, the bacterial species were more abundant and diverse in high temperature period than those in heating period and cooling period; The whole fermentation process was mainly Ascomycota and Basidiomycota at the phylum level; At the genus level, thermophilic fungi such as Dipodascaceae, Talaromyces, Fusarium, Wallemia, Aspergillus, Trichoderma, Thermomyces, Penicillium and Geotrichu were abundant in high temperature period, All of them were the dominant bacteria for the degradation of organic substances such as cellulose; The order of environmental factors on fungal community was C/N > moisture content > EC > E4/E6 > temperature > TN > TC > NO3--N > NH4+-N> pH. The ratio of carbon to nitrogen had the greatest impact on fungal community.