Parameters included in systematic evaluation of staining conditions for CD31, CD8 and FOXP3 in osteosarcoma samples.

Systematic evaluation of more than 200 different antigen retrieval conditions, different blocking conditions, detection systems and chromogens allowed identification of optimal staining conditions for formalin-fixed and paraffin-embedded osteosarcoma samples.* In steps of 3–5°C;** in steps of 5 min;*** in concentrations of 0.1%–10%, adjusted temperatures and various times and combinations.