Parameters included in systematic evaluation of staining conditions for CD31, CD8 and FOXP3 in osteosarcoma samples.
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Systematic evaluation of more than 200 different antigen retrieval conditions, different blocking conditions, detection systems and chromogens allowed identification of optimal staining conditions for formalin-fixed and paraffin-embedded osteosarcoma samples.* In steps of 3–5°C;** in steps of 5 min;*** in concentrations of 0.1%–10%, adjusted temperatures and various times and combinations.
创建时间：
2015-12-02
