File S1 - Positional Assembly of Enzymes on Bacterial Outer Membrane Vesicles for Cascade Reactions

Supporting Figures. Figure S1, Density-gradient fractionation of vesicles. Western blot analysis of different fractions using an anti-His6, anti-OmpA or anti-DsbA serum, respectively. Figure S2, Binding of CelA-DocCT onto wild-type and engineered OMVs. (A) SDS-PAGE analysis of OMVs before and after enzyme binding. (B) CelA activity of the resulting OMVs after binding. Figure S3, Hydrolysis of cellulose by single enzyme binding. Cellulose hydrolysis from the binding of increasing amount of CelA-DocT (CMC) (A), CelE-DocC (PASC) (B) and BglA-DocF (cellobiose) (C). Figure S4, SDS-PAGE analysis of OMVs loaded with all three enzymes. For better resolutions, binding of (A) AT or (B) EC and BF was confirmed using either 10% or 7% SDS-PAGE. Bands corresponding to either INP-scaf3 (107 kDa), AT (53 kDa), EC (90 kDa) or BF (70 kDa) are shown.
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