RNA-sequencing of primary wild-type (WT) and Pla2g4d-deficient (KO) keratinocytes

PLA2G4D is a phospholipase and transacylase expressed in epidermal keratinocytes, and its expression is upregulated in inflammatory skin disorders such as psoriasis. Treatment of primary keratinocytes with proinflammarory cytokines IL17A and TNFa markedly increases Pla2g4d-mRNA expression. To investigate the function of PLA2G4D, we conducted bulk RNA sequencing of primary keratinocytes isolated from wild-type and Pla2g4d-deficient mice and studied transcriptional changes under basal and cytokine stimulation. The sequencing data suggests that PLA2G4D modulates fundamental biological processes including cell proliferation, differentiation, and signaling. Overall design: Primary keratinocytes were isolated from two days old neonatal wild-type (n=5) and Pla2g4d-deficient (n=8) C57BL/6NJ mice. Cells from both genotypes were cultured in the abscence and presence of IL17A and TNFa. Total RNA was isolated and bulk RNA sequencing was performed to study transcriptional changes between groups