Phenotypic variation is observed in the coloboma mutants for both cleft-palate and number of digits.

ACleft-palate severity was recorded for UCD-Co.003 mutant samples (n = 200). In addition to the natural variation of the phenotype, an increase in incubation temperature enhances the severity of the mutation thereby causing an earlier termination of development [4]. See Figure 1 for cleft-palate severity examples.BFoot digit variation was recorded for UCD-Co.003 mutant samples (n = 187). LF refers to the embryo’s left foot, RF refers to the embryo’s right foot. The number preceding the specific side of the leg (right versus left) indicates the number of digits present.