Establishment of breast cancer PDX RNA-sequencing data library [bulk RNA-seq]

Breast cancer is the most commonly diagnosed cancer among women. PDXs (patient-derived xenografts) are similar to cancer cell lines but differ in that they are maintained in a physiological setting as soon as they are isolated from the patient and for subsequent passages. These models are valuable for preclinical trials because PDX models have been shown to closely match their patient counterparts, both in genomic profile and response to treatment. Collection of RNA-expression data from multiple PDX models was performed to generate a library of RNA-sequencing data which may be utilized to compare tumors from different subtypes or treatment groups. Overall design: ER+ and triple negative mammary tumors were grown orthopedically in mice for 8 weeks. Tumor cells from each PDX were profiled by bulk RNA-sequencing to determine differentially expressed genes and gene signatures between models with the goal of furthering the fields of oncogenesis, metastasis and drug resistance.