Single-cell RNA Sequencing Analysis Reveals Cell Landscape and Gene Signatures Associated with Granulomatous Lobular Mastitis

We have employed a single cell sequencing approach using 10x Genomics scRNAseq to study granulomatous lobular mastitis (GLM), a chronic inflammatory breast disease characterized by granuloma formation and recurrent abscesses. GLM is considered an immune-mediated disorder, yet its underlying pathogenesis remains poorly defined. This study is the first to perform single-cell transcriptomic profiling of GLM tissue and provides new insights into the cellular landscape and immune dysregulation associated with GLM. Overall design: Fresh breast tissue samples from 3 patients with granulomatous lobular mastitis (GLM) and 3 healthy controls were surgically obtained. Single-cell suspensions were prepared and subjected to single-cell RNA sequencing (scRNA-seq) using the 10x Genomics Chromium Single Cell 3' v3 platform.