Detection of Ortmanniana ligamentina male mitotype DNA from a mesocosm during a natural spawning event

Data describe a mesocosm study for the detection of Mucket (Ortmanniana ligamentina) female and male mitotype environmental DNA (eDNA) during a spawning event. Water samples (approximately 1L) were collected every Monday and Thursday for 4 weeks during August 2021 from a pond located at Columbia Environmental Research Center containing O. ligamentina and analyzed for the presence of sex-specific eDNA. Parameters described in this data release include qPCR results and associated quality assurance measurements from the mesocosm water samples.