Tang Mitosis/Apoptosis ver. II

Tang Mitosis/Apoptosis ver.II: Dose response of anti-mitotic compounds in human cancer cell lines at 24, 48 and 72 hours to determine effect on apoptosis, mitosis and cell death.

In screening for small-molecule compounds that are effective at killing cancer cells, one-dimensional readout GI50, which is the EC50 value of growth inhibition, is usually used as the only criterion. A major problem with this one-readout approach is that other useful information is discarded, which could be critical for understanding the action of the compounds. In this screen, we use a single-cell-based imaging assay that can report multi-dimensional physiological responses in cells treated with small molecule kinase inhibitors. The data in this dataset are described in PMID: 23788527: Differential Determinants of Cancer Cell Insensitivity to Antimitotic Drugs Discriminated by a One-Step Cell Imaging Assay (J. Biomolecular Screening, 2013). The image analysis algorithm is available at https://github.com/xietiao/Tang_et_al_LINCS_cell_scoring.