Analysis of S. flexneri strains expressing IcsA::BIO Y716* and/or D717* in S-LPS and R-LPS backgrounds.

aP, polar; NP, non-polar.bThe “+++”, “++” and “+” symbols indicate relative band intensities of Western immunoblots of whole cell lysates.c, d“+++”, WT N-WASP recruitment/F-actin comet tail or capping formation; “++”, 20%–80% reduction in N-WASP recruitment/F-actin comet tail or capping formation; “+/−”, 90% reduction in N-WASP recruitment/F-actin comet tail or capping formation; “−”, N-WASP/F-actin tail not detected.e“+++”, WT plaques; “++”, small plaques; “−”, no plaques.