Supplementary Dataset: The Active Metatranscriptome of Marine Pavlovophyceae

Overview This repository contains the processed data, metadata, and interactive reports supporting the transcriptomic and physiological study of four non-axenic marine Pavlovophyceae microalgae (Diacronema virescens, Diacronema lutheri, Exanthemachrysis fresneliae, and Pavlova pinguis). These datasets provide a public resource for investigating gene expression, lipid metabolic pathways, and microbiome dynamics in these economically and ecologically important species. Dataset Contents Quality Control Reports: A MultiQC report (.html) summarizing the quality metrics of the raw sequencing data.Filtered Reads (rRNA Depletion): Ribosomal RNA (rRNA) and non-ribosomal clean reads (.fastq.gz) used for de novo assembly.Processed Transcriptomes: De novo assembled transcriptomes, separated into ribosomal and non-ribosomal catalogs (.fasta).Transcript Abundance Estimations: Kallisto quantification files (.tsv) in Transcripts Per Million (TPM), provided for both ribosomal assemblies (taxonomic profiling) and the clustered non-redundant mRNA catalog (differential expression).Taxonomic Profiling: Interactive Krona charts (.html) illustrating the composition of the non-axenic cultures and their associated microbiomes.Flow Cytometry Metadata: Temporal physiological monitoring data (events/mL) detailing the concentrations of algae and prokaryotes across the culture period. Related Resources Raw Data: Raw RNA-seq reads are deposited in the NCBI Sequence Read Archive (SRA) under BioProject accession PRJNA1453970.Bioinformatics Pipeline: The open-source code used for data processing is available on GitHub: https://github.com/NathanM311/Pavlovophyceae_Transcriptomics