Histone modifications underlie monocyte dysregulation in Systemic Sclerosis patients, underlining the treatment potential of epigenetic targeting [ChIP-seq]

With H3K27ac and H3K4me3 chromatin immunoprecipitation we identified alterations in the chromatin landscape of patients with systemic sclerosis. RNAsequencing data was available for the majority of the subjects included for the ChIPseq analysis and the correlation between the histone marks and gene expression was studied. The samples used in this study are part of the SYSCLASS cohort. H3K27ac and H3K4me3 ChIP-sequencing of CD14+ monocytes derived from peripheral blood (PB) from healthy controls (HC) and limited and diffuse Systemic Sclerosis patients.