Identification of BMP3b as a cardioprotective adipokine released by brown adipose tissue.

Brown adipose tissue (BAT) has metabolic and endocrine effects to the whole-body, secreting molecules that target different tissues. Using transcriptomic analysis from isolated BAT from mice treated with isoproterenol in WT or UCP1-knockout mice, which have disfunctional BAT, we identified Bmp3b as an adipokine produced and secreted by activated BAT. Overall design: To determine the activation of brown adipose tissue from isoproterenol-treated or saline-treated in WT and Ucp1-knockout mice. In these studies mice were implanted with Alzet minipumps for the infusion of isoproterenol (60 mg/kg/day for 3 days) to activate BAT. WT and Ucp1-/- mice with minipumps infusing saline were used as controls. 3 days after minipumps implatantation, mouse brown adipose tissues were isolated to generate respective libraries for genome profiling.