Single cell profiling of lichen planus identifies type I interferon response and potential therapeutic targeting

Cutaneous lichen planus (LP) is an inflammatory skin disease characterized by an interface dermatitis with lymphocyte infiltration and keratinocyte cell-death. Previous studies suggest that LP inflammation is dominated by a type I immune response involving IFN-g but its pathogenesis remains not fully elucidated yet. To investigate the inflammatory mechanisms underlying LP, we performed single-cell RNA sequencing on LP compared with healthy control skins. We demonstrate that LP skin is imprinted by a type I interferon (IFN-I)-rich environment. While all immune cells are imbued with this interferon signature, we highlight unique subsets of inflammatory keratinocytes and fibroblasts highly influenced by IFN-I. Single-cell RNA-sequencing on cutaneous lichen planus and healthy control skins Please note that GSM8513934 sample from GSE277089 was re-analyzed and the re-analyzed data is linked as Series supplementary file. *************************************************************** The table below lists GEO/SRA accessions reused/reanalyzed for this study. ***************************************************************