To verify some of the significant CpGs from the genome-wide analysis, we selected the target CpGs at the warfarin-associated genes. Thereafter, we used pyrosequencing to detect the methylation
For differential expression analysis between HSCR and healthy controls, ECM-related cellular subsets were extracted and analyzed in two comparisons (aganglionic segments vs. distal healthy control seg
For HSCR segments, the normal segment of each subject serves as his or her own control of the aganglionic segment, allowing sufficient power to detect a significant effect of the segment with only a s
