Induction and maintenance of mouse totipotent stem cells by a G-quadruplex unwinder

Using small molecules to induce the establishment of totipotent stem cells is an important and challenging work. Here, we treated mouse embryonic stem cells (ESCs) with a G-quadruplex unwinder to transform them into totipotent cells, called G4TotiSC. We identified the whole cell protein expression levels of G4TotiSC and ESC by proteomic analysis. There were 4 samples in total, and ESC and G4TotiSC had two samples each. We identified a total of 10327 proteins, and 9093 comparable proteins.