RNA sequencing time-course of mesenchymal progenitors during regeneration after notexin induced muscle injury

After tissue injury mesenchymal progenitors undergo a transient proliferative expansion, morphological transition and migration from their perivascular niche. To gain transcriptomic insights into the transition of MPs out of the quiescent state and their role in muscle regeneration, a muscle-injury model was used to activate MPs and follow their contribution to tissue regeneration. tdTomato positive MPs were FACS purified from dissociated muscle at different time points after notexin-induced injury and transcriptomic analysis was performed using RNA sequencing of all samples. Overall design: Transcriptome wide profiling of FACS purified mesenchymal progenitors at various timepoints during regeneration after notexin induced muscle injury.