Gene expression changes in primary human endometrial stromal cells following METTL3 knockdown during in vitro decidualization. Gene expression changes in primary human endometrial stromal cells following METTL3 knockdown during in vitro decidualization

We developed an in vitro model in which primary human endometrial stromal cells (HESCs) were induced to differentiate through treatment with MPA and 8-Br-cAMP. SiRNA-mediated knockdown of METTL3 was performed on HESCs, 6 h prior to treatment of 8-Br-cAMP and MPA. Overall design: To investigate the effect of METTL3 on decidualization, HESCs were subjected to decidualization for 4 days after transfection of siRNA targeting METTL3 (RiboBio, Guangzhou, China; #siG151008053209-1-5; siN0000001-1-5 serving as negtive control) for 6 hours.