Characterization of global transcriptome using Illumina paired-end sequencing and development of EST-SSR markers in sesame (Sesamum indicum L.). Sesamum indicum

Sesame transcriptome was sequenced using Illumina paired-end sequencing technology from 5 tissues (young roots, leaves, flowers, fruits, and shoot tips at the full flowering stage). To minimize the likelihood of systematic biases in transcriptome sampling and Illumina sequencing and make the detection of low-abundance transcripts possible, 3 cDNA libraries from same pooled RNA were prepared and sequenced separately on a flow cell using Illumina HiSeq2000.