RNA Methyltransferase METTL16's Protein Domains Have Differential Functional Effects on Cell Processes

To investigate the cellular role of METTL16, we established HEK293 cell lines over expressing wild-type METTL16 or METTL16 methylation and RNA binding mutations. We then removed endogenous METTL16 from the cells using CRISPR. We then performed gene expression profiling analysis using data obtained from RNA-seq of the various cell lines. Overall design: Comparative gene expression profiling analysis of RNA-seq data for METTL16 knockout HEK293 cells rescued with Wild-type or various mutated versions of METTL16