Developmental competence and <i>eGFP</i> knockin efficiency in Porcine <i>OCT4</i> locus.

Different combination of Cas9, sgRNA and donor plasmid were injected as shown. Cleavage and blastocyst formation rates of each groups are presented. OCT4 targeting efficiency was measured by PCR amplification and sequencing of single blastocysts. Statistical significance was tested using chi-square test. Developmental competence and eGFP knockin efficiency in Porcine OCT4 locus.