Transcriptomic profile of FACS-isolated reactive or SOCS3-deactivated astrocytes from the hippocampus of wildtype and 9-month old APP/PS1dE9 mice

We analyzed the transcriptional profile of astrocytes from 1) WT mice infected with AAV-GFP, 2) reactive astrocytes from 9-month old APP/PSdE9 mice infected with AAV-GFP and 3) de-activated astrocytes from 9-month old APP/PSdE9 mice infected with AAV-SOCS3 We show SOCS3 normalizes the inflammatory profile of APP astrocytes Overall design: Total RNA was extracted from GFP+ astrocytes isolated by FACS from WT and APP/PS1dE9 mice injected with an AAV targeting astrocytes and encoding GFP alone (controls, N=7 WT-GFP, N=4 APP-GFP) or SOCS3 and GFP (N=5 APP-SOCS3, same total viral load). Non GFP+ cells (including microglia, neurons, non infected astrocytes, called OTHER) were analyzed as well, in 3 samples of the control WT-GFP group.