A network including TGFß/Smad4, Gata2 and p57 regulates proliferation of mouse hematopoietic stem/progenitor cells [ChIP-seq]

Transforming growth factor-ß (TGFß) is a potent inhibitor of hematopoietic stem cell (HSC) proliferation. However, the precise mechanism for this effect is unknown. Here, we have identified the transcription factor Gata2, previously described as an important regulator of HSC function, as an early and direct target gene for TGFß-induced Smad signaling in hematopoietic stem and progenitor cells (HSPCs). Interestingly, TGFß-induced Gata2 upregulation is critical for subsequent transcriptional activation of the TGFß signaling effector molecule p57 and resulting growth arrest of HSPCs. Importantly, both Gata2 and p57 are abundantly expressed in freshly isolated highly purified HSCs, demonstrating the relevance of this circuit in HSC regulation within the HSC niche. Our results connect key molecules involved in HSC self-renewal and reveal a functionally relevant network regulating proliferation of primitive hematopoietic cells. To identify TGFß targets downstream of Gata2, we carried out a ChIP-Seq experiment on TGFß-induced Lhx2 cells. Interestingly, there was a large overlap between the GATA2-bound genes and genes differentially expressed after 2h TGFß induction. Overall design: One sample of 1x10^8 cells (treated with 10 ng/ml TGFß for 2h) was sequenced.