Single_cell_transcriptome_of_schistosome_ovary_and_vitellaria_. Single_cell_transcriptome_of_schistosome_ovary_and_vitellaria_

Mice were infected with either mixed-sex cercariae or female cercariae generated by monomiracidia-infection of snails. Six weeks post-infection mature (paired) female worms or immature female worms were collected by portal perfusion of mice infected with mixed-sex cercariae or female cercariae, respectively. Ovaries and vitellaria were isolated from individual female worms following the protocol described by Hahnel et al, 2013 (PMID: 23936567) followed by dissociation into single cell suspensions. The live cells were sorted and prepared for 10X Chromium single cell sequencing (Diaz et al, 2020. PMDI: 33339816). The 10X single cell libraries were prepared and sequenced. The samples for this study are: FUGI_R_D7708903 (bisex ovaries) FUGI_R_D7708518 (single-sex ovaries) FUGI_R_D7733089 (vitellocytes) This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/