Glycolytic acetyl-CoA metabolism maintains histone acetylation for syncytialization of human placental trophoblast stem cells

we comprehensively utilized primary human trophoblast cells, human trophoblast stem cells (hTSCs) and the induced differentiation model, hTSCs-xenotransplanted mice, as well as the methods such as transcriptome, metabolome, and epigenetic analysis to explore the mechanisms underlying the regulation of human placental trophoblast cell fate by glucose metabolism. We found that hTSCs and primary CTBs were metabolically different from differentiated STBs, and glycolysis was highly active in hTSCs and CTBs. Mechanistically, Acetyl-CoA facilitated histone acetylation and thus regulated the expression of genes which are key for syncytialization, inflammation responses, and oxidative stress response.