A novel small molecule targeting oncogenic PELP1 demonstrates anti-tumor activity in wild type and mutant estrogen receptor alpha-positive breast cancer

RNA-seq analyses showed SMIP34 treatment altered the expression of genes associated withEstrogen Response, Cell Cycle and Apoptosis pathways Total RNA from ZR-75 cells treated with or with out SMIP34 was isolated using RNeasy mini kit (Qiagen). For whole-genome transcriptome profiling, 2 libraries (3 replicates each for SMIP34 and control) were generated using a TruSeq Stranded mRNA Library Preparation kit according to the manufacturer’s protocol (Illumina Inc.).