Kir4.1 channels in NG2-glia play a role in development, potassium signaling, and ischemia-related myelin loss.

Single NG2-glia with GFP fluorescence labeling from PDGFRαCreER; mGFP mice was selected and aspirated into a glass pipette from hippocampal acute slices. In brief, cells were picked promptly by micromanipulation and immediately placed in lysis buffer. All NG2 glial cells were collected within 3 h after slice preparation. The selected NG2-glia were processed for single-cell RNA extraction and reverse transcription within 1 h and were subjected to RNA-sequencing.