SMAD2 linker phosphorylation impacts TGF beta 1-dependent gene expression in A549 cells

We investigated the impact of SMAD2 linker phosphorylation (pSMAD2L) on TGF-beta 1 signaling in A549 cells. Data has been published in the British Journal of Cancer under the title “SMAD2 linker phosphorylation influences overall survival, proliferation, TGFβ1-dependent gene expression, and pluripotency-related proteins in NSCLC”. We generated A549 cells constitutively lacking pSMAD2L (A549Lsub) by substituting three serine residues to alanine within the linker domain of SMAD2 (S245A/S250A/S255A). For comparison, we stimulated wild-type and A549Lsub cells with human TGF-beta 1 for 48 hours. Three individual tests were carried out for each stimulation.