RNA-seq in primary Harpegnathos neuronal cultures stimulated with 20E or JH3

We cultured primary Harpegnathos neurons in a medium containing 20E or JH3 and then switched the hormones. RNA-seq was performed 30 minutes, 6 hours, or 24 hours after the switch. To determine baseline expression in the cultures before the switch we also performed RNA-seq on the 6-day-old cultures 30 minues after addition of vehicle (DMSO). At least 3 independent cultures (biological replicates) per condition and time point