Quantitative Analysis of transcriptome in organoids derived from TP190 wildtype(WT) and Williams Syndrome(NSUN-GTF2ird2+/-) human induced Pluripotent Stem Cell

Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived retinal transcriptome profiling (RNA-seq) to microarray and quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods and to evaluate protocols for optimal high-throughput data analysis mRNA profiles of 68-day old wild type (WT) and NSUN-GTF2ird2 +/- cortical organoids