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Mass spectrometry analysis of cleaved Ycf1p in the monomeric and dimeric states

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NIAID Data Ecosystem2026-05-02 收录
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Purified Ycf1p with a C-terminal 3xFLAG tag was obtained from a strain of yeast containing the Pep4p protease. The purified sample was subjected to blue native PAGE. Two gel bands, corresponding to monomeric and dimeric Pep4p-cleaved Ycf1p, were subjected to trypsin digestion.

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2025-05-07
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