Methods are provided to mutate， in a targeted manner， the genome of a plant cell using a double stranded DNA break inducing enzyme. Also provided are plants， in particular Brassica plants that yield
RuSpacer is a database of CRISPR spacer sequences derived from prokaryotes in the ruminant gastrointestinal tracts. This dataset includes 181,023 CRISPR spacer sequences from 9,391 prokaryotic genomes
