Human immature neutrophils as a functional biomarker of systemic vascular pathologies
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https://www.ncbi.nlm.nih.gov/sra/SRP254912
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We used an ex vivo system of murine ER-Hoxb8 neutrophils that phenotypically and morphologically recapitulate neutrophil maturation stages during five days of in vitro culture in the presence of G-CSF. To investigate the development of the ROS system in neutrophils during their development, we compared transcriptomes of D0, D1, D3 and D5 Hoxb8 neutrophils. Overall design: Paired-end sequencing of mRNA of Hoxb8 neutrophils of different in vitro differentiation stages, of two biological replicates, using Illumina HiSeq4000.
创建时间:
2020-11-10



