Regioselective toxicity of 2- and 6-hydroxychrysene during Japanese medaka embryogenesis

Previous studies have shown that exposure to PAHs at critical developmental time points can impair red blood cell development in Zebrafish. The goal of this project is to identify sensitive windows of development in Japanese medaka to oxygenated polycyclic aromatic hydrocarbons. Medaka embryos will be exposed to various concentrations of 2-hydroxychrysene and 6-hydroxychrysene for various durations. Embryos will be monitored daily for presence of mobile red blood cells as well as for viability. Red blood cells will be quantified by staining hemoglobin with ortho-dianisidine and analysis of microscopy images with ImageJ. Sensitive windows of exposure will be identified through partial exposures.