Transcriptome analysis of tubular tissues of focal segmental glomerulosclerosis patients. Transcriptome analysis of tubular tissues of focal segmental glomerulosclerosis patients

Transcriptome comparison of tubular tissues from kidneys with focal segmental glomerulosclerosis (FSGS) and tubular tissues from the unaffected portion of tumor nephrectomies. High-density Affymetrix Human Transcriptome Arrays (HTA) 2.0 were used for the gene expression analysis. Overall design: Focal segmental glomerulosclerosis (FSGS) is a common podocytopathy. Renal tubular tissues from 5 focal segmental glomerulosclerosis patients and 5 controls were manually isolated under a stereomicroscope using 2 dissection needle holders in RNAlater at 4°C.Affymetrix HTA 2.0 microarray was used to perform a global analysis of the gene expression pattern in the tissues.